Protein Tagging Technologies in Cell Imaging and Analysis Event Date: March 25, 2009 12:00 p.m. Eastern Time; 9:00 a.m. Pacific Time Brought to you by the Science/AAAS Business Office     Real Player   Real Media Video (56k/150k)   Windows Media Player   Windows Media Video (56k/150k)     The expression, localization, and degradation of cellular proteins are highly dynamic and exquisitely controlled processes. Although there are a number of approaches to indirectly monitor these processes in fixed cells and cell lysates, tools to study these events in living cells in real time are more limited. The use of cell lines expressing proteins fused to intrinsically fluorescent tags (e.g., GFP) has become routine and the availability of a variety of different fluorescent proteins is driving a wealth of new and exciting research, such as the simultaneous visualization of multiple proteins in a single live cell. In this webinar viewers will learn about the application of current fluorescent protein tagging technologies, as well as a variety of complementary cell imaging approaches including several chemical labeling approaches that combine the flexibility of fluorescent dyes with the specificity and selectivity of genetically encoded imaging tags. During the webinar, our panelists will: Provide an overview of various tagging approaches for cell imaging Discuss how these technologies can be applied in basic research and drug discovery Share data on advancements in cell imaging approaches Answer your questions live   Speakers: Prof. Kai Johnsson Swiss Federal Institute for Technology Lausanne, Switzerland Dr. Kai Johnsson received both his undergraduate diploma in chemistry from Eidgenössische Technische Hochschule (ETH) in Zürich, Switzerland, and his Ph.D. from the same institution, under the mentorship of Steven Brenner. Following his postdoctoral training at the University of California, Berkeley, Dr Johnsson worked for three years in Germany as a Research Assistant at the Ruhr-Universität-Bochum before returning to Switzerland to take an assistant professorship position at the Institute of Chemical Sciences and Engineering with École Polytechnique Fédérale de Lausanne (EPFL). He is currently an Associate Professor at EPFL, researching the development and application of tools to study protein function in vivo and in vitro, as well as the directed evolution of protein function. Dr Johnsson is associate editor of ACS Chemical Biology and a member of the Faculty of 1000 since 2003, as well as being co-founder of Covalys BioSciences, a company commercializing the protein labeling technologies developed in his laboratory. Dr. Jennifer Lippincott-Schwartz National Institutes of Health Bethesda, MD Dr. Jennifer Lippincott-Schwartz attended Swarthmore College, graduating with honors in 1974. After several years of teaching, she turned to laboratory research working with Phil Hanwalt at Stanford University receiving a Master's degree in biology in 1979. Dr. Lippincott-Schwartz then entered the Ph.D. program in biology at Johns Hopkins University studying under the mentorship of Douglas Fambrough. After doing postdoctoral work in the laboratory of Richard Klausner at the National Institutes of Health in Bethesda, Maryland, she established her own lab at the National Institutes of Health, and is now Chief of the Section on Organelle Biology in the Cell Biology and Metabolism Branch. Dr. Lippincott-Schwartz uses live cell imaging approaches to analyze the spatio-temporal behavior and dynamic interactions of molecules in cells. Dr. Lippincott-Schwartz's projects cover a vast range of cell biological topics, including protein transport and the cytoskeleton, organelle assembly and disassembly, and the generation of cell polarity. Analysis of the dynamics of fluorescently labeled proteins expressed in cells is performed using numerous live cell imaging approaches, including FRAP, FCS, and photoactivation. Currently, her research employs photoactivation localization microscopy, called PALM, that enables visualization of molecule distributions at high density at the nano-scale. Dr. Lippincott-Schwartz serves as editor for Current Protocols in Cell Biology and The Journal of Cell Science, and is on the editorial boards of Cell, Molecular Biology of the Cell, and Traffic. She is an active member of the scientific community, serving as a member of the Council for the American Society of Cell Biology and on the Executive Board of the Biophysical Society. She was elected to the National Academy of Sciences in 2008. Prof. Klaus Hahn Department of Pharmacology University of North Carolina Chapel Hill, NC Klaus Hahn earned his Ph.D. in chemistry from the University of Virginia, followed by postdoctoral work in the Center for Fluorescence Research at Carnegie Mellon University. He was an Assistant Professor and Associate Professor at the Scripps Research Institute, where his laboratory pioneered new biosensor designs that revealed precisely timed, localized activations of Rho family GTPases in motility. In 2005, Dr. Hahn became the Ronald Thurman Professor of Pharmacology at the University of North Carolina, Chapel Hill, where he is now focused on systems biology questions, developing new tools to address the spatiotemporal regulation of signaling networks, including genetically encoded caged proteins and artificial kinases. His lab focuses on situations where rapid and precisely localized signaling contributes to cell “decision making”, such as metastasis and neurite outgrowth.   Moderator: Sean Sanders, Ph.D., Commercial Editor, Science/AAAS Sean Sanders did his undergraduate training at the University of Cape Town, South Africa, and his Ph.D. at the University of Cambridge, UK, supported by the Wellcome Trust. Following postdoctoral training at the National Institutes of Health and Georgetown University, Dr. Sanders joined TranXenoGen, a startup biotechnology company in Massachusetts working on avian transgenics. Pursuing his parallel passion for writing and editing, Dr. Sanders joined BioTechniques as an editor, before joining Science/AAAS in 2006. Currently Dr. Sanders is the Worldwide Commercial Editor for the journal Science and Program Director for Outreach.   ___________________________________________________________________________________________________   For the best experience we recommend you use Internet Explorer 6 (IE6) or higher on a Windows-based PC to view this webinar. Please ensure that you are using a computer with a stable internet connection and good bandwidth. To ensure the best possible performance of the webinar we recommend that you avoid using a dial-up connection. Note: please confirm the start time of the webinar in your time zone. A free time zone converter is available at www.timeanddate.com/worldclock/converter.html   Test Your System   Help   Download Real Player   Download Windows Media Player